Fluorescence in situ hybridization (FISH) is a molecular biology technique used to detect and localize the presence or absence of specific DNA sequences on chromosomes. It is widely used in research, clinical diagnostics, and in the study of genetic disorders.
The principle of FISH involves the use of fluorescently labeled DNA probes that bind to complementary sequences of DNA within the cell. These probes are designed to target specific regions of the genome, allowing researchers to visualize the location of these sequences within the cell.
To understand FISH, it is important to have a good grasp of the following concepts:
By mastering these fundamental concepts, you will be well-prepared to understand and apply the principles of fluorescence in situ hybridization in various biological contexts.
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